Metabolic Pathway Engineering for Enhanced PHA Biosynthesis in Prospects of Biomedical Application

Abstract

Modern developments in genetic engineering, proteomics, genomics, and synthetic biology have made it possible to apply all multidisciplinary methods for biochemical pathway modification, protein identification, and prediction. Genetic engineering techniques are applied with increasing time in the industrial production of polymer as it can regulate metabolism of microorganisms and can modify biosynthetic pathways. By modifying the genes involved in the Polyhydroxyalkanoates (PHA) biosynthesis process, PHA production can be increased. Numerous researchers have used molecular modifications of the enzyme. Gene editing has lately made use of CRISPR/CAS9 technology, increasing the expression of proteins. This review highlights the principles and fundamental mechanism behind the genetic engineering and metabolomics strategies to elevate PHA manufacturing using natural biopolymer. The idea is to study the construction of the novel pathways to generate metabolites that are not synthesized or expressed naturally.