Manasi Singh, Yatharth Mishra, Padmini Ganga, Pallavi Kumari2025-11-1820242582-9882http://136.232.12.194:4000/handle/123456789/1566Agri Articles (E-Magazine), Volume 04, Issue 06 (Nov–Dec 2024) Agri Articles Editorial BoardThis article discusses how genetic manipulation—particularly CRISPR-Cas9—enables precise editing of DNA to improve crop traits. It explains the CRISPR mechanism, including Cas9-mediated DNA cutting and repair, and outlines applications in agriculture, medical science, ecology, and livestock improvement. It highlights ethical issues such as off-target effects, biosafety concerns, GMO release, biodiversity reduction, and regulatory challenges. The article concludes with future prospects including improved crop yields, enhanced resilience, and broader applications such as space exploration and climate change mitigation.en-USCRISPR-Cas9Genetic ManipulationGene EditingTransgenic CropsCrop ImprovementGMOsGenome EngineeringAgricultural BiotechnologyBook chapter